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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene is observed in many tumors, which results in chromosome instability, and so may well contribute to tumorigenesis. This protein was uncovered to affiliate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a significant purpose in advertising mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are already explained. Two more splice variants ended up documented but detailed nucleotide sequence facts is just not yet readily available.
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Blocking peptides are peptides that bind exclusively into the goal antibody and block antibody binding. These peptide commonly is made up of the epitope regarded from the antibody.
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This guide outlines further probable optimization of cellular and immunoassay parameters to make sure the very best effects are...
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This guideline outlines further feasible optimization of cellular and immunoassay parameters to make sure the best possible outcomes are...
Antibodies bound to the blocking peptide not bind towards the epitope around the goal protein. By comparing the staining with the blocked antibody vs . the antibody by yourself, you can see which staining is distinct.
Goal Information and facts Cyclin E belongs into the really conserved cyclin family members, whose members are characterised by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins show distinct expression and degradation styles which lead on the temporal coordination of each and every mitotic event. Cyclin E varieties a posh with and features like a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S changeover. This protein accumulates for the G1-S stage boundary which is degraded as cells progress as a result of S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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